Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: Both setups, hyperspectral camera setup (left part) and microscope filter-wheel setup (right part), are sketched. Each setup can be divided into three parts: illumination unit (top row), camera and sensors (middle row), and data processing unit (bottom row).

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: Microscopy

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: The specific sensor responses for (a) all 16 bands of the 4 × 4 spectral pattern of the used visual hyperspectral snapshot camera and (b) all 25 bands of the 5 × 5 spectral pattern of the used NIR hyperspectral snapshot camera presented by the manufacturer XIMEA, Germany.

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques:

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: The general calibration workflow for both setups. The calibration steps (a)–(c) as well as (e) and (f) are needed for both setups, whereas step (d) is only performed for the hyperspectral camera setup. Calibration step (a) corrects the internal setup effects of the used sensors and lenses. The sensor noise is reduced in step (b). In step (c), the measured sensor data are converted to physical reflectance values. Step (d) ensures that the specific spectral information is achieved and crosstalks are eliminated. The steps (e) and (f) are necessary for visualization and validation of the spectral behavior of both setups.

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: Biomarker Discovery

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: These curves substantiate the need of the described calibration chain. (a), (b) The two reference spectra (□, magenta tile and ⋄, cyan tile) of the hyperspectral camera setup. (a) The raw data, the filled markers represent the raw sensor counts, and the unfilled markers represent the sensor counts corrected with the specific correction matrix C from Eq. (4). (b) The corrected and white balanced data (the dotted lines show the reference data of the color chart). The unfilled markers represent the calculated reflectance data I res using Eq. (2) without applying Eq. (4) and the filled markers represent the fully corrected data I final , which is the fully corrected physical reflectance. For the microscope filter-wheel setup, (c) shows the same behavior of the “magenta” tile. (d) The pixel counts (dotted line) are completely different to the HSI camera setup but the reconstructed spectrum (black line) behaves as the reconstructed line of the HSI camera setup and the reference data, which shows the reconstructed magenta spectrum of both setups with the specific reference data of the color chart as dotted line.

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: Microscopy

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: Calibration results of all analyzed color spectra with the filter-wheel setup. The reference data, plotted as dotted lines, could be fully reconstructed for all curves. The reconstructed data points have a distance of 20 nm in the visual range and a distance of 50 nm in the NIR. The results are comparable to the results of the hyperspectral camera setup ( Figs. 9 and ).

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques:

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: A selection of eight out of the 24 measured reference spectra acquired with the hyperspectral camera setup used with LED surgical light. The reference information is plotted as dotted lines. The results show the same behavior for all scanned spectra as with the Xe illumination setup, cf., Fig. 9 .

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: Selection

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: Calibration results of all 24 analyzed color spectra with the hyperspectral snapshot camera setup and Xe illumination. The reference data, plotted as dotted lines, could be fully reconstructed for all curves. In the region of NIR ( λ > 840 nm ), the deviation d between reconstruction and reference is increasing, but the results are comparable to the results of the microscope filter-wheel setup ( Fig. 8 ) as well as the HSI camera setup with LED illumination, see Fig. 10 .

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: Microscopy

Journal: Journal of Medical Imaging

Article Title: Validation of two techniques for intraoperative hyperspectral human tissue determination

doi: 10.1117/1.JMI.7.6.065001

Figure Lengend Snippet: The reconstructed RGB calculation using the same measured hyperspectral snapshot data and the CIE color matching functions. The measured raw pixel counts are used for RGB calculation in (a) which results in a false greenish RGB appearance, whereas in view (b) the corrected reconstructed spectral data are used for RGB calculation, which results in a realistic RGB appearance.

Article Snippet: The alternative HSI setup consists of up to two hyperspectral snapshot cameras of XIMEA, Germany.

Techniques: